D-Luciferin: Benchmark Firefly Luciferase Substrate for B...

2026-01-20

D-Luciferin is a membrane-permeable bioluminescent substrate essential for sensitive intracellular ATP quantification and promoter-driven gene expression monitoring. The compound enables non-invasive tumor burden assessment and pharmacodynamics studies in vivo, establishing itself as the gold standard for bioluminescence imaging. This article details its biochemical mechanism, evidence benchmarks, and workflow integration, with a focus on reproducibility and translational impact.